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Questions related from Adam Wei Jian Soh
Hi guys, I have recently optimized my protein expression from BL21 cells and I have a query on the types of storage methods that can be used. I have seen the use of 0.02% sodium azide and 50%...
28 October 2014 4,672 5 View
Hi, I recently purified an eukaryotic protein from BL21 cells. Upon examining the fractions, it seems that this protein is found predominantly in the insoluble pool. As such, I need some advice on...
12 October 2014 6,856 7 View
Hi guys, I would like to know if there is any recommended websites that I can rely on for isoelectric point estimation? I used the one by expasy and it seems that the value deviates by at least...
30 July 2014 3,394 18 View
Hi guys, I am considering to perform crispr knockout but am not sure if this will be effective on both alleles. The DSB pathway is preferred since it is more controlled but before I attempt it, I...
29 July 2014 8,400 13 View