Adam Wei Jian Soh

4 Questions 17 Answers 0 Followers

Questions related from Adam Wei Jian Soh

How do we prepare purified proteins for long term storage?

Hi guys, I have recently optimized my protein expression from BL21 cells and I have a query on the types of storage methods that can be used. I have seen the use of 0.02% sodium azide and 50%...

28 October 2014 4,678 5 View

A query on protein purification and refolding from insoluble fraction

Hi, I recently purified an eukaryotic protein from BL21 cells. Upon examining the fractions, it seems that this protein is found predominantly in the insoluble pool. As such, I need some advice on...

12 October 2014 6,859 7 View

How can I estimate a protein isoelectric point and its implication on purification?

Hi guys, I would like to know if there is any recommended websites that I can rely on for isoelectric point estimation? I used the one by expasy and it seems that the value deviates by at least...

30 July 2014 3,398 18 View

What is the efficiency of Cas9 crispr knockout in diploid organisms?

Hi guys, I am considering to perform crispr knockout but am not sure if this will be effective on both alleles. The DSB pathway is preferred since it is more controlled but before I attempt it, I...

29 July 2014 8,403 13 View