Adam Wei Jian Soh

3 Questions 10 Answers 0 Followers

Questions related from Adam Wei Jian Soh

How do we prepare purified proteins for long term storage?

Hi guys, I have recently optimized my protein expression from BL21 cells and I have a query on the types of storage methods that can be used. I have seen the use of 0.02% sodium azide and 50%...

28 October 2014 4,622 5 View

A query on protein purification and refolding from insoluble fraction

Hi, I recently purified an eukaryotic protein from BL21 cells. Upon examining the fractions, it seems that this protein is found predominantly in the insoluble pool. As such, I need some advice on...

12 October 2014 6,807 7 View

How can I estimate a protein isoelectric point and its implication on purification?

Hi guys, I would like to know if there is any recommended websites that I can rely on for isoelectric point estimation? I used the one by expasy and it seems that the value deviates by at least...

30 July 2014 3,330 18 View