Adam Schlobohm

3 Questions 2 Answers 0 Followers

Questions related from Adam Schlobohm

RCAG PCR and DNA Extraction: Is there a way to tell if your DNA Extraction was unsuccessful?

I have been troubleshooting a RCAG PCR reaction for a couple weeks now intermittently between other tasks. Every time I run it with my extracted DNA, I do not see any bands after I run the gel....

30 January 2017 8,847 3 View

Is there any way to tell if the serum or other media additives (FBS, EGF, BPE, etc.) used in cell culture are of bad quality?

Recently in the lab that I currently work in, we have had trouble growing our cell culture. We use a 3D matrigel insert cell culture method to culture mouse alveolar lung epithelium (specifically...

29 November 2016 1,414 3 View

Has anyone struggled getting a viable number of cells from dissociating frozen human lung tissue?

I am currently trying to dissociate normal human lung tissue that has been frozen @ -80C for about three weeks to set up for MACS sorting and upon my first round I obtained an extremely low number...

16 November 2016 1,978 0 View