Adam Richardson

4 Questions 7 Answers 0 Followers

Questions related from Adam Richardson

How to image Antibody/Receptor internalization?

Hello, I am interested in determining whether the binding of our antibody to a cell membrane receptor causes the receptor to be internalized or not. We have data that suggests the antibody...

21 January 2020 8,630 6 View

In vitro crosslink negative and positive controls?

Hello, I am looking to do in vitro crosslinking using formaldehyde and DSS to determine if two recombinant proteins of interest form a heterodimer. We also anticipate homodimers. I originally...

09 January 2020 2,959 2 View

What is causing PCR smearing and optimization issues?

Hello, I am currently having issues optimizing my PCR reaction. I am unsure if I am getting amplification when using 250 ng of template due to my template only control having a similar band. My...

09 January 2019 5,171 11 View

I would appreciate some help with calculating and plating HeLa cells from a 100mm plate to a 96 well plate.

Hello, to continue with a protocol I need to do some optimization for a 96 well plate. One of the tests I need to do is have cells be at 60% and 80% confluency and judge which is better for...

24 June 2017 4,058 4 View