How can i insert my 7kb plasmid in Agrobacterium strain by freeze/thaw shock transformation?

I have a plasmid pGpd-GFP arround 7 kb. I successfully inserted my inserts (0.6 kb) into to plasmid by phusion pcr followed by cloning with E. coli. Now I would like to transform my agrobacterium...

02 February 2019 7,602 9 View