My protein runs different after a few days in SDS-PAGE

Hi, I am working on a protein that runs in SDS-PAGE around 55kDa after Nickel purification (buffer 20 mM Tris pH8.0, 300 mM NaCl, 5% glycerol and 200 mM Imidazole), then I run through SEC in 20 mM...

01 January 1970 4,835 5 View