Abosede Elesinnla

3 Questions 3 Answers 0 Followers

Questions related from Abosede Elesinnla

Which enzyme is best to amplify a 3.8kb GC rich viral DNA?

I want to amplify a 3.8kb GC rich DNA for sequencing and cloning. I need a polymerase enzyme that will do a good job. Please help.

01 November 2016 7,948 5 View

Stab culture medium preparation using LB agar?

I like to preserve some bacteria culture using stab culture technique but I like to know if I need to add more sodium chloride to my normal LB broth which contains 5g/L of sodium chloride

01 September 2016 7,350 3 View

Self ligation of a linearized vector?

I used a TOPO TA kit which contained a linearized vector of 3.9kb to ligate my DNA of about 3.8kb. After transformation and plating on LB agar containing X-GAL I got quite a number of white...

07 August 2016 9,912 3 View