Abishai Dominic

3 Questions 4 Answers 0 Followers

Questions related from Abishai Dominic

I see two populations in SSC/FSC plot when I did flow analysis of RAW264.7 cells. Can someone explain if I have did the right gating to select cells?

HI! I culture RAW cells in DMEM and did flow analysis. When performed flow analysis I saw that there a two populations in the SSC/FSC plot. I believe that the other population was debri. I have...

10 October 2017 676 4 View

Should RAW264.7 cells be serum starved to perform ELISA upon LPS activation to measure IL-1beta and TNF-alpha cytokines?

I am trying to measure secreted cytokines TNF-alpha and IL-1beta from RAW264.7 cell lines after LPS stimutaion. I am unsure whether the cells should be serum starved for performing ELISA from the...

09 September 2017 751 4 View

Can RAW 264.7 macrophage cell line be grown in a-MEM ? How do we decide which media to grow this cell line?

I have used DMEM to grow macrophages before. I would like to know if a-MEM can be used instead of DMEM. What is the advantage or disadvantage of each of the media?

07 July 2017 797 0 View