Abirami Santhanam

2 Questions 5 Answers 0 Followers

Questions related from Abirami Santhanam

Have any one used Bio Rad turbo transfer for transferring high molecular weight protein around 450kDa?

Hi, I am trying to use fast turbo transfer for transfer of my protein of mol.wt 450kDa. Is it possible to do by turbo transfer? I am trying to get the condition for perlecan western blots. Any...

30 December 2014 6,544 7 View

What is the best way to detect extra cellular proteins like perlecan and nidogen from cultured fibroblast using western blot?

Right now I am using 1% NP40 containing lysis buffer to collect the lysate from rabbit corneal fibroblast cells.

23 September 2014 7,338 4 View