Abigail Rubiato Cuyacot

3 Questions 6 Answers 0 Followers

Questions related from Abigail Rubiato Cuyacot

How do you check the efficiency of Arabidopsis root protoplasts isolation?

I have isolated successfully protoplasts from Arabidopsis roots following the published article (Ryu et al., 2019). I am just wondering if there is a way to somehow check its efficiency because I...

12 February 2020 2,302 3 View

What is/are the fastest methods to select homozygous transformants?

I've crossed transgenic Arabidopsis, a mutant (dominant mutation) and reporter lines. I am wondering if there's faster way to select homozygous from T2 generation aside from using resistance...

07 December 2016 4,533 20 View

If anyone already tried to use the powder 1-MCP (1-Methylcyclopropene) to block C2H4, can I put it directly on media?

I am trying to treat Arabidopsis seeds with 1-MCP to block ethylene (competitive inhibitor). But I don't know how to exactly use the 1-MCP powder since it will then release its gas whenever mix...

02 November 2016 5,613 6 View