Why do DH5a strain of E. coli transformed with plasmid containing E2 Crimson as reporter gene under a high copy number origin give dark blue colonies?

I'm getting dark blue colonies and lawns when I'm plating E. coli cells transformed with plasmid containing E2 Crimson as the gene of interest under high copy number origin of replication and...

12 May 2023 7,733 2 View