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Questions related from Abhishek Bastiray
So I am currently titrating a ligand (A, 12 µM) which is a dimer (in syringe) to a monomeric protein in cell (B, 61 µM). The experimental conditions are 20 mM Sodium Phosphate, 100 mM NaCl, and 2...
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I purified my protein of interest using Ni-NTA chromatography. The protein was eluted but it eluted with a yellowish brown color which I have experienced before. I believe it was due to excess DTT...
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I have been purifying a His-tagged protein first using Ni-NTA chromatography and then Gel Filtration. After purifying using Ni-NTA, the protein seems to be precipitated after keeping the protein...
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