Abhishek Bastiray

2 Questions 2 Answers 0 Followers

Questions related from Abhishek Bastiray

Low stoichiometric constant (N value) from ITC. Any ideas to further optimise it?

So I am currently titrating a ligand (A, 12 µM) which is a dimer (in syringe) to a monomeric protein in cell (B, 61 µM). The experimental conditions are 20 mM Sodium Phosphate, 100 mM NaCl, and 2...

30 October 2023 1,786 1 View

How do I avoid protein precipitation after Ni-NTA purification?

I have been purifying a His-tagged protein first using Ni-NTA chromatography and then Gel Filtration. After purifying using Ni-NTA, the protein seems to be precipitated after keeping the protein...

14 August 2023 662 7 View