Abhisek Mondal

2 Questions 7 Answers 0 Followers

Questions related from Abhisek Mondal

Is it possible to isolate transmembrane protein ?

Hi, I have been trying to incorporate a solubilized transmembrane toxin protein into eukaryotic cell membrane. Upon incubation with vesicles the protein form pores and ruptures the vesicles as...

11 November 2016 6,504 3 View

Why does this membrane protein not bind to His-affinity column ?

Hi, I'm trying to purify a 31kD eukaryotic membrane protein(N-ter His tagged), expressed in E. coli. Detergent solubilization is performed to get this protein out from inclusion bodies and...

12 December 2015 9,309 12 View