Abhinav Kanwal

6 Questions 15 Answers 0 Followers

Questions related from Abhinav Kanwal

Why are specific proteins or genes only expressed in the specific body part?

My question is as so many proteins/genes are present in a cell or a tissue how and why specific ones are only expressed?

05 May 2014 2,822 1 View

PBST or TBST for western blot?

What is good for western blot PBST or TBST? Sometimes people use only TBS and PBS also, and still results good with no background.

10 October 2013 350 7 View

What are differences in anti-fade mounting media in IHC/ICC?

IHC protocol

09 September 2013 7,151 2 View

What are the various techniques to see how much your protein gets phosphorylated?

I want to see the how much my protein gets phosphorylated. But its phospho-antibody is not available in the market. Is there any method except doing western blot with phospho antibody of same target?

08 August 2013 5,091 2 View

RIPA Buffer in protein lysis?

Generally I use RIPA buffer for protein lysis for western Blot from tissues and cells. But I have noticed it interferes while doing estimation of proteins by Bradford's method. However I also...

08 August 2013 1,827 3 View

How can I solve a problem in maintaining H9C2 cells?

I'm having a problem in maintaining H9C2 cells for a long duration. After 2-3 splits cells become unhealthy as black particles start coming. Also sometimes this problem happens from the beginning....

08 August 2013 4,554 4 View