Abhinav Hatwar

2 Questions 1 Answers 0 Followers

Questions related from Abhinav Hatwar

I am using Millipore 100,000 kDa cutoff filter for the protein purification and concentration still i am able to see lower molecular weight bands..?

I have purified my protein with the Ni-NTA column. I am trying to concentrate and remove the lower molecular weight impurities through Millipore Amicon filters with a cutoff of 100,000 kDa....

13 February 2025 1,557 5 View

How to avoid self Ligation even after phosphatase treatment..?

I am trying molecular cloning of vector 5.717 kb with 3.792 kb of the gene with a single digestion. However, I am giving phosphatase treatment, but I am still getting a self-ligated vector after...

03 March 2024 9,146 3 View