Abhilasha Ojha

6 Questions 15 Answers 0 Followers

Questions related from Abhilasha Ojha

Does nicotine actiavtes muscarinic receptors?

Nicotine activates neuronal and muscle receptors. Does it also activate muscarinic receptors?

06 September 2017 226 1 View

Does it make any difference if we use different host cells for transfecting same channel?

I am working with muscle type nicotinic acetylcholine receptors. I make transient transfection in HEK -293 cells. Does it make any difference in channel function specially in terms of kinetics ,...

04 December 2015 9,383 4 View

What is the way to do out side out patching?

I want to do out side out patch in HEK cells having nicotinic acetylcholine receptors. Normally we get good amount of current from whole cell. we also get succeded in outside out patch but its...

29 October 2014 4,261 4 View

Can anyone give advice regarding transient expression?

Transient expression of a4b2 neuronal nicotinic Ach receptors in HEK 293 cells. We tried to transiently transfect HeK Cells with neuronal a4b2 nachr+CFP, but found very low expression. When we...

04 April 2014 8,237 5 View

Which is much better for expression of alpha 2 beta 4 neuronal receptors in ooytes, injecting mRNA or cDNA?

We want to perform outside out patching in oocytes. Therfore, we injected mRNA in 1:10 (alpha:beta) ratio. Patching was done after 3 days of injection and we applied 50 micromolar of Ach solution,...

04 February 2014 8,285 2 View

Is it possible to have stable cell lines (HEK-293) containing muscle nicotinc acetylcholine receptors having alpha, beta, delta and epsilon subunits?

We are working on muscle nAchR and have plasmids for 4 subunits i.e. alpha subtype 1, beta subtype 1, epsilon and delta. In addition to this we have a CFP plasmid. Untill now we were doing...

17 July 2013 2,276 3 View