Abhilash D. Pandya

4 Questions 4 Answers 0 Followers

Questions related from Abhilash D. Pandya

How to confirm specificity of Ab?

I am doing some IHC experiments and I have some problems while staining gut biopsies. There is faded brown shade on epithelium and tubular glands around Goblet cell. Now I need to know if this is...

08 August 2013 9,229 1 View

What could be an alternative to 0.1% HCl and Ammonium hydroxide for post washing step of a hematoxylin stain?

The hematoxylin stain is giving extra color to the samples and I want to avoid that. We don't have Ammonium hydroxide and thus I am seeking an alternative option.

11 November 2012 7,735 29 View

Can we dilute Hematoxylin prior using for IHC?

I get very high staining with hematoxylin. I want to reduce it. How can I possibly reduce it? I use Ammonium hydroxide and water washing steps afterwards.

11 November 2012 4,793 4 View

Why do we use Citrate buffer in Antigen retrieval step during IHC?

What is the use of Citrate buffer in Antigen retrieval?

11 November 2012 2,478 8 View