Abhijit Babaji Shinde

6 Questions 22 Answers 0 Followers

Questions related from Abhijit Babaji Shinde

Fellow researchers, can you please share your views about "Factors that can impact/affect blood glucose levels in mutant mice"?

I have some mutant mice which show elevated blood glucose levels as compared to controls. So I just wanted to take valuable opinions of all of you about which factors relating mainly to beta-cells...

19 August 2016 3,571 5 View

Chemical fluorescent probe for monitoring mitochondrial hydrogen peroxide?

Apart from MitoPY1 is there any other probe that can selectively detect mitochondrial hydrogen peroxide?

26 June 2014 8,642 1 View

What are the techniques apart from western blots, qPCR and immunohistochemistry to characterize a knockout mouse model?

I am completely aware of use of western blots, qPCR and immunohistochemistry for characterizing a knockout mouse model. I just wanted to know, whether there is any other approach that can be used?

26 June 2014 2,464 2 View

Which is best method for isolation of islets from mouse pancreas?

I came across two procedures wherein the first collagen digestion steps are same but one uses handpicking for purification while the other uses ficoll gradient. I was just wondering whether...

21 March 2014 1,353 20 View

What is the best method for transfecting primary mice hepatocytes?

I am interested in looking at the ROS in the cytoplasm and mitochondria using some ro-GFP probes for which I have to transfect primary hepatocytes with these plasmids. I have already tried...

27 August 2013 4,512 9 View

Which is the best hepatic (non-cancerous) cell line for studying the effect of gene knock out using siRNA?

Currently I am investigating the effects of knocking down Pex-5 from hepatocytes using primary hepatocytes from WT and liver specific Pex-5 KO mice livers. As these primary hepatocytes are...

21 August 2013 4,883 14 View