Abhijit Mitra

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Questions related from Abhijit Mitra

Is it necessary to seed uniform number of cells for different intervals of time (such as 24 Hr, 48 Hr, 72 Hr) in case of MTT assay?

Hi I intend to perform MTT assay for 24 Hrs, 48 Hrs, and 72 Hrs drug treatment. Is it ok to seed different numbers of cells for each plate (24 Hrs, 48 Hrs, and 72 Hrs)? Or is it mandatory to seed...

22 August 2024 1,493 1 View

Is it necessary to change the medium every 24 hours after drug treatment in cell line study and provide fresh drug to the cell?

I am keeping my cell line for drug treatment and monitoring its effect at 24 hrs, 48 hrs and 72 hrs? Should I change the medium every 24 hrs and give fresh drug treatment or just leave the medium...

02 May 2024 10,039 3 View