Hi everyone. So recently I have done qPCR and I got this weird amplification plot. However, the melt curve looks fine. Can anyone explain this?

I isolated RNA from NHBE cell line using qiagen minikit. The perform cDNA synthesis (1000 ng) and run qPCR using SYBR green with GAPDH primer (200 nM).

16 December 2024 4,248 4 View