Which lysis buffer should I use for HEK293T cells, which I have transfected for purification of a non-secretory (approx 200 kDa) protein?

Hi Everyone, I am a beginner at eukaryotic(HEK293T) protein expression. I am trying to purify a HIS tagged 200 kDa viral polyprotein using pCDNA3.1 vector. I am not sure which lysis buffer should...

12 January 2021 2,141 3 View