Abhigyan Sengupta

4 Questions 44 Answers 0 Followers

Questions related from Abhigyan Sengupta

What will be the influence on the fluorescence signal in a confocal FCS setup, if the pinhole is removed?

Hi all, To improve the signal to noise ratio in FCS I am planning to remove the pinhole from the confocal microscope. I am interested to know if 1. Anyone has done this before? Do you know any...

06 June 2018 454 3 View

What is the free amino acid concentration in cell ?

Is there any study of free amino acid concentration in mammalian cells ?

08 August 2015 6,013 0 View

Why doesn't the fluorescence spectrum look like Gaussian distribution?

If you closely look at any fluorescence emission spectrum you will find the both side of the emission maximum is not equal in area. The left hand side of the emission maximum appears with a...

03 March 2014 1,014 13 View

What is the fate of Maleimide-Thiol adduct at low pH ?

What is the fate of Maleimide-Thiol adduct at low pH? Is Maleimide-Thiol adduct stable at low pH (pH=2)? If the pH of the medium is decreased after the formation of the Maleimide-Thiol adduct,...

01 January 1970 5,469 5 View