Abel Flores

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Questions related from Abel Flores

Is it acceptable to use two sets of FL wavelengths (ex/em) for my analyte and internal standard?

In my current bioanalytical method development project, I achieved optimal separation between my analyte (A) and internal standard (IS) using HPLC with fluorescence detection. However, the...

16 January 2023 9,577 11 View

Do I need to validate method, or simply submit an amendment doc, if I will adjust inj volume, from 80 to 50 uL during a clinical sample study?

The current HPLC autosampler only allows a maximum injection volume of 50 uL, while the validated bioanalytical method states 80 uL injection volume. This shall be applied to an on-going clinical...

14 May 2021 7,222 5 View