Abeeshna Nambidi

3 Questions 8 Answers 0 Followers

Questions related from Abeeshna Nambidi

I did PureLink™ HiPure Plasmid Extraction using 25ml culture thrice. I got very bad ratios and DNA concentration. Why is it so?

I did this 3 times and couldn't see any pellets after adding isopropanol and ethanol. I did nanodrop twice. The concentration of DN and ratios were not at all satisfactory Where am i going...

10 August 2024 9,833 9 View

Will using direct E. coli liquid stock culture from VectorBuilder for plasmid extraction affects the yield and ratio?

I did this twice. I couldn't see any pellets after adding isopropanol the 1st time The second time, the yield and ratio was really bad.

07 August 2024 8,445 4 View

I did PureLink™ HiPure Plasmid Extraction using 25ml culture. I got really low DNA and very bad ratios in nanodrop. can someone help troubleshoot?

I don't have 12000 g centrifuge. I did 3220g 4 times. It took me some time to add lysate to the column due to debris. According to the protocol, the total volume after adding Elution Buffer and...

06 August 2024 4,747 2 View