Has anyone observed co-purification of a 55 kDa protein from E.coli lysate on Ni-Sepharose?

All three recombinant proteins varying in sizes (17 kDa, 26, Kda and 29 kDa) are co-purifying with a 55 kDa protein. Two proteins were extracted from E.coli [whole cells pellet] with Buffer A (50...

23 August 2017 9,844 5 View