Abdou Nagy

4 Questions 20 Answers 0 Followers

Questions related from Abdou Nagy

Can I store my cells for few days at 4 C before FACs analysis?

I want to ask if it is possible to store my cells 24 hours post transfection at 4 C for 4-5 days (Non-fixed) just in normal media as one of my conjugated antibody shipment is delayed and will be...

07 July 2019 3,843 5 View

How do I solve cloning problem of unstable gene into PHW 2000 plasmid?

I have cloned all eight genes from H9N2 virus into PHW2000 plasmid successfully. All prepared midi plasmids were verified correct with Sanger sequencing except PHW-HA plasmid which gave me...

07 July 2018 7,651 5 View

How can I purify certain virus from mixed infection?

If I get lung tissue sample from naturally-infected chicken. The sample was PCR positive against H9N2 and NDV viruses. How can I purify NDV from original sample using polyclonal NDV antiserum?

08 August 2017 3,552 5 View

What is the best solution to increase concentration of low copy plasmid using mini-prep?

I am working with expression plasmid (2.6 kb) and I ligate it with an insert of 15.186 kb to it. I grew my positive colony (identified by colony PCR) on LB (6 ml+ selective antibiotic overnight...

08 August 2017 5,200 4 View