Abdelmalik Ibrahim Khalafalla

3 Questions 19 Answers 0 Followers

Questions related from Abdelmalik Ibrahim Khalafalla

Could anyone give me advice how to correctly annotate my sequence within Sequin program of GenBank?

I have 3 nt sequences a virus gene and am using Sequin to submit the sequences to the GenBank. I tried more than two online software or ORF finders to do the annotation before manual entering of...

08 October 2015 8,468 3 View

Why do I get late Ct value in SYBR green qPCR?

In an effort to develop a triplex probe qPCR I started uniplex qPCR with SYBR green. For two targets the test performed well. For the 3rd target I got late Ct values several times. Primers, probe...

08 June 2014 7,919 4 View

Is it possible to develop a qPCR to detect all known Papilloma viruses using generic primers?

I already used the Pan-papilloma primers (FAP59/64) for detecting camel papilloma virus in skin nodules that worked perfect. Now I like to develop a qPCR assay, but as papilloma viruses are...

13 April 2014 8,951 4 View