5 Questions 16 Answers 0 Followers
Questions related from Abdalla Mohamed
Hi, I'm trying to generate p53/p16 double knockout cell line. I managed to knockout p53 using Crispr. Now, I can't manage to knockdown/out p16. I have tried so far 5 different Crispr targeting...
06 June 2017 7,705 4 View
I'm trying to package pBabe-KRAS, pBabe-beta-catenin and pBabe-PIK3CA retroviral constructs. So far, I have no luck with beta-catenin or PIK3CA although there is no issues with KRAS or empty...
09 September 2016 3,844 3 View
I'm struggling to package a plasmid and want to give phoenix cells a try?
08 August 2016 7,903 0 View
I have two plasmids on the same backbone. Both of them have eGFP. The only difference is that one plasmid has 3x flag before the insert and the other doesn't have. When I transfect HEK293T cells...
01 January 2014 2,544 2 View
I have sub cloned some constructs. I added a flag tag before the insert. I have confirmed the constructs by PCR, diagnostic digestion and sequencing. Now, I'm trying to validate them using western...
10 October 2013 2,084 6 View
