4 Questions 9 Answers 0 Followers
Questions related from Abdalla Mohamed
Hi, I'm trying to generate p53/p16 double knockout cell line. I managed to knockout p53 using Crispr. Now, I can't manage to knockdown/out p16. I have tried so far 5 different Crispr targeting...
23 June 2017 6,810 3 View
Hello, Does anyone know if it's possible to use this construct to clone two gRNAs targeting two genes? If yes, is there a protocol or reference? Thanks, Abdalla
09 November 2016 1,384 3 View
I'm trying to package pBabe-KRAS, pBabe-beta-catenin and pBabe-PIK3CA retroviral constructs. So far, I have no luck with beta-catenin or PIK3CA although there is no issues with KRAS or empty...
13 September 2016 8,622 3 View
I have sub cloned some constructs. I added a flag tag before the insert. I have confirmed the constructs by PCR, diagnostic digestion and sequencing. Now, I'm trying to validate them using western...
28 October 2013 979 5 View
