Abbas Mohajeri

2 Questions 7 Answers 0 Followers

Questions related from Abbas Mohajeri

Does anyone have experience with cloning using pET21a and host is E.coli?

I want to clone a human gene in the pET 21 a, in Ned1 site. This site has ATG and my sequence has ATG. As result there be 2 ATGs. Is it necessary that I have my sequence ATG or should I delete it...

02 February 2014 8,300 2 View

Anyone have experience cloning a human gene in pET-21a and transferring to E.coli?

I want to clone a human gene in pET-21a and transfer to E.coli. I cloned the gene in the Nde1 site and it’s sequence is CA/TATG My question is, does the ATG of Nde1 act as a start codon and as a...

02 February 2014 3,881 5 View