Abbas Ismail

3 Questions 1 Answers 0 Followers

Questions related from Abbas Ismail

Why small scale high-throughput result is not reproducible in 500ml flask?

have anyone ever encountered a situation that small scale expression (0.3 ml 96 well plate) is not reproducible when repeated in 500ml flask? the conditions of both expression is the same, i.e:...

06 June 2017 5,953 3 View

Any suggestion of software /web server to develop a model to describe relationship between three variables?

Hi, I'm doing directed evolution on one enzyme. the changes in amino acids affect the activity of the enzyme. from the analysis of mutants library, hydrophobicity and percentage helix are two...

01 January 2017 3,973 3 View

Why did my recombinant protein not secrete into medium?

I am doing secretion of recombinant protein using E.coli. Before this, my protein was being secreted outside the cell into the medium and periplasm. When I want to repeat the same experiment after...

07 July 2014 2,361 1 View