Aathithya Rengarajan

2 Questions 1 Answers 0 Followers

Questions related from Aathithya Rengarajan

Trobleshooting in Mamallian transfection?

I'm trying to do transient transfection on snu638 cell line. I'm using flag tag plasmid for transfection , initially it got worked. But if I try to repeat it it is not working I tried different...

12 April 2023 9,299 0 View

I'm doing western blot for protein of 160KDa. Is it sufficient to do transfer at 100mA for 2 hours using nitrocellulose membrane?

Thanks in advance for your replies.

08 April 2015 134 5 View