Aasim Habib Wani

3 Questions 6 Answers 0 Followers

Questions related from Aasim Habib Wani

Can anyone suggest vectors for metagenomic library for bioactive molecules?

I am doing research on screening of metagenomic library for enzymes and bioactive molecules. I want to know about the expression vectors for preparing functional metagenomic library.

01 July 2015 7,700 3 View

Can intranasal or intratracheal routes be used to study the immune response in calves against live P. multocida B:2?

When the animal is challenged intranasaly or intratracheally will there be any clinical signs in animal? I challenged calves intranasally with 5ml of inoculum containing 109 cfu/ml but observed no...

17 June 2015 7,277 3 View

How can I concentrate 34 mg of recombinant protein from 200 ml of 100mM immidazole buffer?

Hello, I have eluted my protein in 200mM imidazole Na phos buffer, on storage the proteins precipitated and could not be concentrated, so I had to redo my expression again. In the next elution I...

02 February 2015 649 10 View