Aaron Owji

2 Questions 7 Answers 0 Followers

Questions related from Aaron Owji

Used aqueous phase of equilibrated phenol chloroform isoamyl in RNA extraction - can anyone help?

When performing an RNA extraction (to remove DNase for rt PCR) I shook the phenol chloroform before using. I thought the two organics were immiscible and needed mixing prior to use. Turns out I...

09 September 2013 2,272 3 View

Whether to quantify cDNA prior to Real Time PCR, and different dCt values on standard curve?

I am fairly new to Real Time PCR and have some questions regarding the protocol. Question 1 - When should input RNA and/or cDNA be normalized? I was told that one should normalize the RNA...

08 August 2013 9,168 17 View