Aaron Martin-Alonso

4 Questions 4 Answers 0 Followers

Questions related from Aaron Martin-Alonso

Is it possible to sequence LAMP products?

I have carried out a LAMP reaction, and I have obtained the typical ladder pattern. I wanted to know whether I can cut one of those bands and sequence it, trying with the different primers I have...

03 March 2017 9,694 3 View

How should I treat missing values in microRNA expression analyses?

I am performing microRNA expression analysis (750 miRNA) and, as was expected, I have many missing values (consequence of Ct values above or below cut-off, low amplification scores, or simply...

04 April 2016 6,869 1 View

Should we use multiple comparison corrections in miRNA expression analysis?

We are doing miRNA expression analysis using Taqman OpenArray panels. We are analyzing aproximately 500 miRNAs. I wanted to know if we must do Bonferroni or Benjamini-Hochberg in order to adjust...

03 March 2016 814 2 View

How can I get a final Ct value when using two technical replicates in miRNAs expression analysis?

I am doing microRNA expression analysis, and we have used two technical replicates (platform QuantStudio 12k Flex). I know three technical replicates is the optimum but trying to balance technical...

02 February 2016 7,923 4 View