The melt curve of a molecular beacon works fine against a complement oligo but not against a clear amplicon on a AB7500. Any ideas?

AB7500 Titrations of MgCl2, dNTPs, and probe have not resolved the issue.  Again, there's a clear melt peak with the probe against a complementary oligo but the amplicon (gel and sybr green...

01 January 1970 5,777 3 View