Aalap Parikh

2 Questions 4 Answers 0 Followers

Questions related from Aalap Parikh

What is the upper limit on plasmid size that can be transformed into chemically competent TOP10?

Hello, I am currently working on a cloning a 6 kb insert into 14 kb vector by Gibson Assembly. Following heat shock transformation into chemically competent TOP10 cells, I had multiple positive...

19 December 2024 5,399 16 View

Why does soybean half-seed transformation use 1/10x B5 salts during co-cultivation?

I was curious as to why modern soybean half-seed protocols use 1/10x strength B5 salts during the co-cultivation step rather than full strength B5 salts? Also, are the B5 vitamins added at full...

08 May 2024 3,983 0 View