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Questions related from A. Murphy
The protein shown below is a 173 kD, monomeric, glycosylated human membrane protein. I previously thought the best expression host to use was insect cells however given that insect cells...
24 July 2017 9,670 3 View
In recombinant protein production, is glycosylation of the protein product affected solely by choice of expression host, or does choice of vector (e.g. pUC18/19 {Escherichia coli} vector vs pPICZ...
24 July 2017 3,536 3 View
Why do we use Saccharomyces cerevisiae for insulin production as opposed to Picha pastoris?Surely the hyperglycosylation of S. cervisae would trigger an immunogenic response in humans, P. pastoris...
24 July 2017 7,953 2 View