A. C. E. Draper

3 Questions 3 Answers 0 Followers

Questions related from A. C. E. Draper

Do lentiviruses containing DNA simply integrate this in to host cells?

This is probably a daft question; I have cloned in a gene of interest to a lentiviral expression vector and successfully made lentiviral particles that easily transduce HEK cells producing the...

03 December 2018 3,734 1 View

Does anyone have a good protocol for fractionation of nuclear from cytoplasmic proteins?

My current protocol is: Procedure: Note: All centrifugations should be done at 4°C. 1) Scrape cells on 10 cm plates using 5 ml of cold PBS plates immediately and place in 15 ml falcon tube. 2)...

29 November 2016 5,503 3 View

Does a low TU/ml in a specific lentivirus equate to a low transduction efficiency?

There is quite a range in the TU/ml between different batches of virus that I make. If I make 4 different gene variants at a time using the same plasmid backbone, packaging and envelope plasmids...

13 June 2016 8,457 9 View