In my case, where I have predicted 3D structure using Rosetta software and it seems that for all atoms B-factor was zero. Is there any way to generate models with Bfactor like experimental structures? Is it necessary to use native PDB file as template to generate model 3D structures?

Model PDB file looks like this..

ATOM 1 C5' G 1 2.322 1.218 0.000 1.00 0.00 C

ATOM 2 C4' G 1 3.794 0.984 0.238 1.00 0.00 C

ATOM 3 O4' G 1 3.990 0.349 1.524 1.00 0.00 O

ATOM 4 C3' G 1 4.478 0.016 -0.716 1.00 0.00 C

ATOM 5 O3' G 1 4.744 0.587 -1.987 1.00 0.00 O

ATOM 6 C1' G 1 5.165 -0.424 1.498 1.00 0.00 C

ATOM 7 C2' G 1 5.725 -0.344 0.077 1.00 0.00 C

ATOM 8 O2' G 1 6.692 0.695 0.018 1.00 0.00 O

ATOM 9 N1 G 1 6.901 -5.110 2.469 1.00 0.00 N

ATOM 10 C2 G 1 7.593 -3.978 2.112 1.00 0.00 C

ATOM 11 N2 G 1 8.923 -4.105 1.997 1.00 0.00 N

ATOM 12 N3 G 1 7.024 -2.806 1.884 1.00 0.00 N

ATOM 13 C4 G 1 5.685 -2.855 2.044 1.00 0.00 C

ATOM 14 C5 G 1 4.899 -3.931 2.398 1.00 0.00 C

ATOM 15 C6 G 1 5.522 -5.183 2.639 1.00 0.00 C

ATOM 16 O6 G 1 4.998 -6.255 2.963 1.00 0.00 O