How to do mono formylation of N,N-diethyl-3-methoxybenzamide?

I have attempted the formylation of the above mentioned compound using a very well reported protocol using secondary Butyl Lithium and TMEDA at - 78 degrees Celsius. I have done it quite a few...

08 February 2021 2,793 2 View

What is the minimum number of cells required for fluorescence in situ hybridization?

I have to perform a FISH experiment with limited number of cells and I am trying to estimate the minimum number of cells required to do FISH. Cells are from mammalian cell line. I failed in doing...

12 January 2021 7,887 1 View

Can you give me some Information about the generation of ROS in marine surface water?

Reactive oxygen species is a very new term i think

23 December 2020 8,416 4 View

Can someone suggest me a wear detection method for a pipe which goes under intense heat and pressure at intervals?

I am looking for a wear detection method for a pipe that goes under intense heat and pressure for short instances at intervals. this process has an average cooldown time of 8 seconds between each...

08 October 2020 3,776 2 View

Single-cell cloning from SkBr3?

Did anyone tried single-cell cloning from SkBr3 cell line. I am failing in growing cells post single cell seeding in wells. Any help is appreciated

27 September 2020 8,762 4 View

What to do when RT PCR becomes positive again after 15-20 days of 1st sample positivity?

We are getting some patients who were initially positive of RT PCR for Covid. But in retesting after 15-20 days or even 20 days after those patients remain still positive. Is it for dead RNA...

17 August 2020 9,552 9 View

How to calculate % sequence identity using Clustal W?

I am calculating % epitope conservation for different mammalian antibodies against zebrafish. I have performed the Clustal W for antigenic peptide used in raising the antibodies against the...

05 August 2020 6,399 5 View

What is the Source of Harmonic Power ?

Consider a sinusoidal supply at fundamental frequency as shown, with a series inductive reactance leading to a bus. Let a diode rectifier load be connected to the bus. The switch Sw for resistive...

24 June 2020 8,175 27 View

What is the best book on Game theory from the perspective of Computer Science and Engineering?

I do not know much about game theory. I am finding a book where different protocols and algorithms of computer science have been analyzed using the concept of game theory.

17 May 2020 4,774 7 View

How can a PhD in interdisciplinary fields can benefit a researcher in job market?

I am a PhD scholar of Humanities and my research is broadly connected to Anthropological studies and framework. I have also published with an Anthropological journal of global repute and looking...

10 May 2020 4,202 1 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Open Source IoT Simulators supported by Machine Learning?

Dear Research Gaters, Let me first add context to the question. I am looking to simulate the waste management system and resources of a city. The hardware devices I need to simulate include,...

21 February 2021 2,333 4 View

How to correctly choose the number of solution intervals when preforming harmonic analysis in ANSYS?

Good day, I design the rail-wheel geometry and the harmonic frequency ranges between 20-10kHz with 500Hz solution intervals and the results makes sense. Most of the time on the related literatures...

19 February 2021 3,190 3 View

Anyone know how to contact Raymond Streater?

He is emeritus in the mathematics department of King's College London. The email address listed on their web site is not functional. The department has no way of physically contacting him due to...

15 February 2021 5,529 1 View

How to create the context-mechanism-outcome configuration (CMOc) in the realist methodology?

In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...

08 February 2021 2,792 1 View

How to generate MATLAB code from ANFIS model?

Dear scholars, I was wondering how can I generate MATLAB code of an ANFIS (FIS) model? I need to integrate my ANFIS (FIS)/Fuzzy logic system into another MATLAB code to render optimizations. Do...

04 February 2021 6,098 4 View

Is there any publication about the effect of basketball and volleyball on body height?

Many people believe that some stretching sports with vertical jump such as basketball and volleyball can increase the body height. On the other hand, others do not agree with this argument. The...

02 February 2021 281 6 View

Qiime2- No reads passed the filter. How to set the trunc value?

Hi, I am working on some 16S sequencing data, and seems some of them are low quality. I am not sure how to set the trunc value in dada2. qiime dada2 denoise-paired \ --i-demultiplexed-seqs...

30 January 2021 6,213 3 View

Please ,How can I correct this kind of error?

27 January 2021 4,815 5 View

In which situation the morphological characters are a synapomorphy?

If the morphological characters which I am studying occur in three species of one section, but there are five species in the section, can I describe this morphological character synapomorphy for a...

14 January 2021 5,361 2 View