i used gblock for trimig 730 sequences then i used this file for phyml online software(http://www.atgc-montpellier.fr/phyml/) but there is an error:
Unexpected error:
Unexpected error while computing Log-likelihood . Err: Problem with species 1A_rm.1's sequence. . Observed sequence length: 732, expected length: 732. . Type enter to exit.
please give me solution.
even after elimination object sequence, it shows problem with another sequence.
also one time select 350 sequences and program was run but when i work by 350 sequence and 50 new sequence, software shows error.
must number of sequences was more than number of characters?in my analysis number of characters is more than number of sequences.
PhyML - ATGC: Montpellier Bioinformatics
www.atgc-montpellier.fr/phyml/
PhyML - ATGC: Montpellier Bioinformatics
www.atgc-montpellier.fr/phyml/usersguide.php?type=phylip
I used the following method to achieve a 700-sequence phylogeny tree
-Alignment by mafft(https://mafft.cbrc.jp/alignment/server)
-Trimming by gblock(http://molevol.cmima.csic.es/castresana/Gblocks_server.html)
-convert fasta format to phyliip by BioEdit
-Analysis by phyml (http://www.atgc-montpellier.fr/phyml/)
I noticed, problem is in output file from bioedit then I used online software (http://phylogeny.lirmm.fr/phylo_cgi/data_converter.cgi) for convert format data after that I could analyze my data by phyml.
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