Are there any other plant species can be transformed by flower dipping?
Can someone provides me the most suitable protocol?
I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...
02 March 2021 8,437 2 View
I am looking for supporting studies discussing literature limitations on the relationships between digital capabilities, market orientation, brand perception and business performance. Also, a...
14 February 2021 7,457 5 View
Hello there.. I've recently submitted my master thesis in marketing. As a reseacher, I now know that the purpose of a master degree is to know academic research tools and how to do and write an...
09 February 2021 6,692 9 View
Dear colleagues I have the following questions concerning the safety of mRNA vaccines targeting the spike protein of SARS-Cov2: 1) The vaccine elicits antibodies which block the peptide domain...
04 February 2021 721 6 View
Also it's effects on plants.
22 December 2020 2,318 3 View
Dear all, I have right now a problem with measuring the charge mobility of different polymers. I have built different types of thin layers OLEDs using different thickness types of polymers. In...
01 December 2020 5,673 4 View
Hello I am trying to do flowcytometry for BAL fluid so i inject1ml of PBS into the trachrea and aspirate back(this done three times) . to calculate the cell count in bal i use a cell counter...
16 November 2020 2,367 2 View
I want to test chitosan nanoparticles as antimicrobial against pseudomonas How to use resazurin microtitre assay????
08 November 2020 9,533 2 View
I received a bottle of PMMA in solution with no information about which solvent was used, so I started using acetone to dilute the original solution. I had not experienced any consequences or...
03 November 2020 4,065 4 View
Hello, I am trying to model a four point bending for RCC beam using abaqus CDP model. I used displacement control type. The issue is whenever I change the value of the applied displacement, it...
25 September 2020 4,395 2 View
I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...
01 March 2021 8,079 2 View
Covid 19 Pandemic and digital transformation
22 February 2021 3,351 5 View
Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...
21 February 2021 7,260 4 View
Hello everyone, currently, I'm trying to do RNA extraction from seeds of Arabidopsis. I'm using the protocol below: RNA isolation from siliques, dry seeds, and other tissues of... I use about...
17 February 2021 7,583 4 View
I am trying to amplify the TGA1 (Transcription factor 1 ) of Arabidopsis ,which is 1107 bp. I have treated the plants with Salycylic solution over night and extracted the RNA and make cDNA. I...
17 February 2021 7,802 2 View
I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...
16 February 2021 8,519 5 View
Hello, I'm checking if the log-tranformation of time series data is a necessary step; We all know the aim of such transformation, but in every study (in time series analysis) we found the authors...
15 February 2021 5,655 14 View
Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...
15 February 2021 9,563 6 View
Hi, all I am using some protocol for protoplast production from Fusarium graminearum (Fg), but I need them in a huge amount. Sometimes the protocol worked well but most of the time failed....
14 February 2021 2,286 4 View
Hello, I am planning to do a transformation, and on the protocol it says "Scrape a 50 ml volume of yeast from the YPAD plate using a sterile inoculating loop or sterile toothpick". But I can...
14 February 2021 6,247 3 View