Which is the best possible method to isolate spinal cord intact from laboratory mice?

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Sedat Develi

Hi, check this article

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3770983/

Nia Lopez

Thanks Sedat,I havent tried ejection yet,let me see.

Refik Kanjhan

Dear Nia,

I used to prepare brainstem-spinal cord preparations for recording from nerve roots from lumbar, cervical and brain stem using dorsal approach. I used to that in late embryonic and newborn mice, and you will need a stereomicroscope and a special bath or dish in which you can pin the mice down. For details, see the following paper.

best wishes, Refik

Article Glycinergic and GABAergic Synaptic Activity Differentially R...

Hi Refikl,

Is it necessary that we should get the entire spinal cord intact for immunohistochemical staining?

Dear Sedat,

Thank you so much for the article.Ejection technique works!It just took me 5 min to get the spinal cord:)

Though the ejction technique worked,both my histo and RNA results were not good.ct values were in the range of 30s.Is there any solution for the same?