Hi
im trying to calculate mg GAE/g extract value. Im trying to use the following equation:
(mg GAE/g dry extract)=C* V/M
Where C is mg/ml (based on gallic acid calibration curve)
V is ml volume
and M is mass
I initilay dissolve my dried extract in dionized water then take 40 ul(0.1g/ml) and add to water , FC and Na2Co3 making a total volume of 4 ml.
my question is V is whether the total amount of reagents used during the assay or only the volume of extract itself (what i mean is that during the preparation my total volume including FC reagent , soudioum carbonate and water is 4 ml but my extract is only 40 microliter.Which one should i take into account
many thanks
Check this link for possible answers
https://www.researchgate.net/post/Can_anyone_help_me_calculate_Phenol_content_of_Plants_extracts2?_tpcectx=post_detail_recommendations&tpr_view=62fcbe16-3f8e-4507-95e4-6ff36b94d536_2&_trid=246632b8-6d4a-46a2-aad9-f1ce9729c150_2
V is equal to total volume including Fc , bicarbonates and water plus the volume of your extract which is 0.04 ml
Check this link for possible answers
https://www.researchgate.net/post/Can_anyone_help_me_calculate_Phenol_content_of_Plants_extracts2?_tpcectx=post_detail_recommendations&tpr_view=62fcbe16-3f8e-4507-95e4-6ff36b94d536_2&_trid=246632b8-6d4a-46a2-aad9-f1ce9729c150_2
Hi Amir
I am also working on antioxidant potentials of medicinal plants. The 'V' is the final volume of the reaction mixture after adding extract and all reagents. and the same thing is being followed in all other calculations of flavonoid, frap.
All the Best.
hi thx for replies i realized what my error was. i didnt set the calibration concentration based on my total volume of experiment which was resulting in comparing apples with oranges.
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