Take a spatula full of lyophilized hen egg lysozyme and dissolve it in a few drops of 0.1M Sodium Acetate pH 4.7, make sure all the protein powder dissolves, add more powder or buffer to yield a dense solution.

You can do the next step in a PCR tubes ("batch crystallization") or some fancy crystallization plates ("vapor diffusion"). Splitt that solution and use 5M NaCl to adjust the aliquots to ~0.25-1.0M sodium chloride without diluting them too much. Seal the experiment and wait a few minutes to a few days, there will be crystals.

Hello

you can refer to these articles and I hope that they will be useful.

Toward Further Understanding of Lysozyme Crystallization: Phase Diagram, Protein−Protein Interaction, Nucleation Kinetics, and Growth Kinetics

Protein Crystallization for X-ray Crystallography

Crystal growth studies of lysozyme as a model for protein crystallization

https://www.jenabioscience.com/crystallography-cryo-em/get-started/crystallization-of-model-proteins/co-401-lysozyme-solution

Improvement on the crystallization of lysozyme in the presence of hydrophilic ionic liquid

HEWL is one of the proteins that can be easily grown using different crystallizating methods from batch and ceiling method to vapour diffusion and counter diffusion. The easiest indeed is the batch method that can be configured to be the ceiling method and result in the growth of high resolution crystals. Prepare 20 mg/mL HEWL in 0.1M sodium acetate pH 4.6 and 8-10 gm% NaCl in the same buffer and mix both parts 1:1 after each is completely dissolved... Set the protein solution still and avoid vigorous shaking to avoid unwanted denaturation... Gentle shaking only without forming bubbles.

After mixing protein and precipitant solution set the crystallization solution still in 18-20 celcius degrees and wait for Crystals!

You may want to read this, Article Practical course "Protein crystallography", Rijksuniversitei...

mother liquor: 1,4 M NaCl, 50 mM sodium acetate, pH=4.7 using hanging

drop vapor diffusion method

30 – 40 mg/ml lysozyme in 50 mM sodium acetate, pH=4.7 mixed in a drop

with a reservoir solution at a 1:1 ratio.