08 August 2017 4 4K Report

What techniques could be used to identify the number of zinc atoms present within a protein?

Some techniques that I could think of are PAR-PMPS assay, crystallography, and atomic absorption.

I would really appreciate any suggestions.

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How to purify oxidation prone proteins?

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Is there a native PAGE for tricine gels?

I would like to perform a native PAGE of my small protein ~6.5 kDa to see its physiological oligomeric state. Normally, I use tricine SDS-PAGE to separate the proteins but I am not sure whether...

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Why is my expressed protein differs in size in different host cells?

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04 May 2016 8,212 7 View

How do you separate Ni-NTA beads from cell lysate?

Dear all, I accidentally mixed Ni-NTA beads with E. coli cell lysate but has a problem with separating them.  Is there any way to separate them so that I can regenerate Ni-NTA beads for...

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Is 50% ammonium sulfate considered high (in concentration)?

Dear all, I am using ammonium sulfate precipitation for my assay and my protein started to precipitate at 50%. Therefore, is 50% ammonium sulfate considered concentrated? Thank you very much

31 December 2015 4,652 8 View

To obtain protein structures, why should wavelength equal to interatomic distances?

In crystallography, including other approaches to resolve protein structures, is there any explanation why the wavelength that we use must equal to interatomic distances? Is there any way to...

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Is it possible to separate antigen-antibody complexes without denaturing the proteins?

I'm not quite sure whether this is practical or not but I am looking for a method to separate antigen-antibody complexes without denaturing them, especially the antigen (which is a protein)....

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I am having problems with biotinylation consistency, does anyone have any suggestions/advice?

I am biotinylating my monoclonal antibodies at the moment. However, I have biotinylated two batches of them and the reactivity & background(non-specific binding) seems to be...

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