Does addition of IPTG before and after lowering induction temperature affect protein expression levels?

Dear all, I would like to know whether addition of IPTG before (37 degC) and after lowering induction temperature (16 degC) affect protein expression levels? *add IPTG before = add IPTG when 37...

09 October 2018 9,796 7 View

How to purify oxidation prone proteins?

Dear all, I am currently purifying a small protein which contains 5 cysteines. The purification flowis: Ni-NTA -> His tag removal by TEV cleavage -> remove TEV protease by Ni-NTA column...

02 March 2017 1,940 4 View

What causes protein to accumulate in inclusion bodies after change competent cell batch?

Dear all, I am using BL21(DE3)pLysS to express my protein (as indicated by the red arrow). Both pictures showed significant protein expression pre- and post- induction. The only difference between...

09 October 2016 4,775 6 View

Is there a native PAGE for tricine gels?

08 September 2016 555 4 View

Why is my expressed protein differs in size in different host cells?

04 May 2016 8,100 7 View

How do you separate Ni-NTA beads from cell lysate?

03 April 2016 6,086 7 View

Is 50% ammonium sulfate considered high (in concentration)?

31 December 2015 4,472 8 View

To obtain protein structures, why should wavelength equal to interatomic distances?

In crystallography, including other approaches to resolve protein structures, is there any explanation why the wavelength that we use must equal to interatomic distances? Is there any way to...

10 November 2015 6,268 4 View

Is it possible to separate antigen-antibody complexes without denaturing the proteins?

02 March 2015 3,173 21 View

I am having problems with biotinylation consistency, does anyone have any suggestions/advice?

01 February 2015 2,904 7 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

How to include original NMR data in a manuscript?

hello. i am junior researcher. I received a comment from a reviewer that he could not find NMR data in my submitted paper. I had included the NMR graph with explanation and formula to calculate...

27 February 2021 734 4 View