Hello everyone,
I'm working on an experiment in which we need to know phenolic contents of marine angiosperms (Posidonia oceanica and Cymodocea nodosa). In addition to this variable many others measurements will made, some of which have to be made with fresh material, so it would be very desirable to delay in time (days or weeks) analysis of the phenolic content. This analysis would be done by extraction in methanol (80%) and subsequent determination with the Folin-Ciocalteu method. Conservation methods used (in both marine and terrestrial plants) vary widely in literature including drying (at room T ° or oven), lyophilization, freezing in liquid nitrogen and conservation at -80 ° C, or directly conservation at -20º.
What conservation treatment would be best suited to ensure optimal measure of phenols in time?
many thanks
Hello,
You would surely find some answers to your questions in this recent review:
http://link.springer.com/article/10.1007/s11101-014-9362-4
Assessing the response of plant flavonoids to UV radiation: an overview of appropriate techniques
Julkunen-Tiitto et al. Phytochem Rev (2015) 14:273–297
DOI 10.1007/s11101-014-9362-4
Francoise
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