Is there any effect of heat, if I will keep the test tubes in hot water bath for evaporation of extra ethanol during phenol or flavonoids extraction?

Whether there is any effect of heat on phenol and flavonoids concentration if the samples are kept in hot water bath for evaporation of ethanol?

10 November 2020 4,096 4 View

Is it possible that a protein band appears on a western blot but not detected on ELISA?

I am working on recombinant protein expression in E. coli. Recently, I thought I had expressed a certain protein successfully as I could see a band of the expected size - 17 KDa (this band was...

14 October 2020 5,144 4 View

How many GCMs exist currently? Which are the most prominent ones among them?

General Circulation Models or Global Climate Models are over 40 when I went through a research article long back. I would like to know how many GCMs are currently in use. Names of them aren't what...

03 August 2020 2,789 5 View

Is it possible to fix a co-culture of bacteria and human cells in 96 well plates and use the plates at a later point for flow cytometry analysis?

I am co-culturing bacterial strains with human cells (WBCs) and there are about 3000 different bacterial strains. The idea is to carry out flow cytometry experiments with these samples (in 96 well...

09 July 2020 3,106 5 View

Is groundwater content considered in SM2RAIN algorithm?

I have come across SM2RAIN algorithm and can't help but wonder about groundwater content. I couldn't notice its inclusion in the algorithm. Is it something I've missed or I need to refer further?...

09 June 2020 5,272 6 View

How to do regridding using MATLAB?

I have a batch of netCDF files of precipitation data at 30km * 30km resolution. I attached a sample file of it. I want to convert them to 10km * 10km resolution using MATLAB. I tried using...

12 May 2020 6,007 6 View

How to build alignment files for homomeric and heteromeric proteins using Modeller?

I am building protein structures for a list of query proteins via homology modelling using Modeller. In some cases the template is a homomeric protein and in others its a heteromeric protein....

01 April 2020 6,576 3 View

Although every year hundreds of new rice varieties are being released? In reality, still the popular rice varieties are those relessed decades back?

For example Swarna, Pooja, etc. (released more than 30 years back ) are dominant rice varieties in eastern India, all the surveys depict.

05 March 2020 9,052 3 View

What are the uses of extreme values in Hydrological aspect?

For the research work I'm working on, I came across usage of extreme values while studying about deriving IDF curves for future climatic scenarios. What kind of results can I produce with extreme...

27 January 2020 4,145 4 View

What's your stand on the argument that - "without human insights, data and the hard sciences will not meet the challenges of the next decade"?

16 January 2020 2,871 3 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

How to improve and implement WBAN MAC and Radio modules under Castalia 3.2 ?

Hello researchers, Hello Castalia3.2 researchers I am a beginner with Castalia, I work on optimization of RFID systems used in wireless body networks. I simulated a WBAN with 10 nodes and one...

28 February 2021 400 1 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

How to increase coupling range (distance between reader and sensor coils) in LC resonance circuit?

Hello great researchers, I am working on a wireless pressure monitoring project where sensing part is actually an LC resonance sensor and I am using a planar coil as a reader to get wireless...

11 February 2021 4,148 5 View

I am looking for a microbend optical sensor design...can anyone please provide me some idea about it??

I am working on a landslide model...i need an optical sensor to determine the amount of pore pressure and other values..i am thinking of using a microbend sensor. Can anyone please provide me with...

11 February 2021 5,911 2 View

How to choose the Hidden Layers Number and RBM Size in a DBN network?

Hello, When using a Deep Belief network (DBN) for images classification, how could we choose the number of hidden layers (number of Restricted Boltzmann Machines) and nodes (RBM size) in a hidden...

09 February 2021 4,618 5 View

Do there exist stand-alone permanently in-ground soil-moisture sensors with an installed GSM module, for outdoor use?

Am searching for a stand-alone permanently in-ground soil-moisture sensor with an installed GSM module, for outdoor use. The idea is for the sensor to send a moisture reading via a cellphone text...

04 February 2021 2,047 4 View

How can I check how different treatments maintain a certain pattern?

Hi there, I have a network where I have used different aggregation methods for its nodes. Now I want to see if these aggregation methods changed their ranking of importance and their importance...

28 January 2021 5,513 3 View