Diabetes was induced by a single intraperitoneal injection of STZ (60 mg/kg) in 0.1 mol/l citrate buffer (pH 4.5) to the overnight fasted rats. Non-diabetic rats were injected with a similar volume of citrate buffer alone. Two days after the STZ injection, fasting glucose levels were determined in a blood sample taken from the tail vein. Diabetes was confirmed when blood glucose levels were at least 250 mg/dl for 2 consecutive days.
What are fasting plasma leptin levels in non-diabetic and STZ-induced diabetic rats?
Kindly find the paper attached below.
Good day
Article Serum Leptin, Adiponectin and Tumor Necrosis Factor-α in Hyp...
The numbers for leptin looked fairly low in the publication cited by Ali so I did a quick search and found that indeed, others have reported much higher values (as is the case of human numbers). For example, here is a study published 2006:
Leptin secretion after a high-fat meal in normal-weight rats: strong predictor of long-term body fat accrual on a high-fat diet
Am J Physiol Endocrinol Metab 290: E258 –E267, 2006;
The above study finds levels of leptin in normal rats at 6.9±0.3ng/ml (as opposed to ~200pg/ml in the previously quoted publication).
The following publication has some relevant information from humans:
http://www.ncbi.nlm.nih.gov/pubmed/20484473
It may be important to dig a little deeper before relying on absolute values - compare the methodology used for quantitation etc.
Best,
Dear Dr. Tausif Alam,
The papers are helpful.
Thank you,
Best regards,
Kazemi
Dear,
Actually I needed the fasting plasma leptin levels in STZ-induced diabetic rats.
Thanks anyway.
Best regards,
Kazemi
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