Do we need to test the enzymatic activity of the supernatant and the wash too? and what is the differences between recovery and residual activity?
The activity retention (RAE %) was calculated following the equation 1:
(RAE %) = 100 x (Immobilized enzyme activity in U/mg) / (solublized enzyme activity in U/mg) ................(1)
The immobilization efficiency, IE (η%), was determined by equation 2 (Menoncin et al., 2009), where P0 was the protein content in the enzyme solution (mg) and P1 was the amount of protein adsorbed on the supports (mg). P1 was estimated by the difference between total protein content added to immobilization process and the protein content washed from the supports.
IE (%) = 100 × (P0/P1) .................(2)
Best regards,
Faheem
Please go through the following reference:
Boudrant J, Woodley JM, Fernandez-Lafuente R. 2020. Parameters necessary to define an immobilized enzyme preparation. Process Biochem. 90:66-80.
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